Ultrasonic Cell Disruption Separation And Purification Equipment 40Khz

Hangzhou Shengtu Technology Co., Ltd. is one of the leading manufacturers and suppliers of ultrasonic cell disruption separation and purification equipment 40khz in China. Welcome to buy high quality customized products at low price from our factory. Contact us for more details.

 

 

 

40Khz ultrasonic cell fragmentation technology utilizes high-frequency ultrasound to generate cavitation effects, i.e. bubbles formed and collapsed in the liquid, which generate shear forces and shock waves on the surface of the cell membrane, ultimately leading to cell membrane rupture. The ultrasonic cell breaker is used for various types of cells, including bacteria, yeast, animal cells, plant cells, etc. It can effectively destroy cell walls and membranes, and obtain relatively pure intracellular substances.

The control of parameters such as ultrasonic frequency, power, and action time can control the crushing effect.

 

 

1. Extract intracellular enzymes, proteins, nucleic acids and other biological macromolecules
2. Preparation of bacterial lysates for vaccine production
3. Separate intracellular organelles such as mitochondria, lysosomes, and many others
4. Preparation of cell membrane layer for biofilm research

 

 

 

1. Frequency selection:
In general, it is more appropriate to use ultrasonic frequencies of 20-50 kHz for plant cells.
Low frequency (such as<20 kHz) can easily cause organelle damage, while high frequency (such as>100 kHz) has poor fragmentation effect.
2. The relationship between frequency and power:
High frequency ultrasound usually requires lower power to produce sufficient crushing effect.
Lower frequency ultrasound requires higher power to achieve the same crushing effect.
3. Frequency and selectivity:
Different frequencies of ultrasound may additionally be extra conducive to detrimental unique mobilestructures.

Lower frequencies are greater conducive to detrimental mobilephone walls, whilst greater frequencies are greater conducive to adverse phone membranes.
4. Frequency and temperature rise:
Higher frequency ultrasound can more effectively avoid excessive heating of the sample.

However, decrease frequency ultrasound typically generates a huge temperature upward push and requires cooling measures.

In summary, it is essential to pick out a sensible frequency when the use of ultrasound to destroy plant cells. It is typically endorsed to pick from the vary of 20-50 kHz and optimize frequency and strength parameters primarily based on unique goal cells.

 

 

       

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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