Ultrasonic Cell Disruption Separation And Purification Equipment 40Khz

Hangzhou Shengtu Technology Co., Ltd. is one of the leading manufacturers and suppliers of ultrasonic cell disruption separation and purification equipment 40khz in China. Welcome to buy high quality customized products at low price from our factory. Contact us for more details.

 

 

 

40 kHz ultrasonic cell disruption technology relies on high-frequency ultrasound to trigger cavitation. During this process, microbubbles form and implode within the liquid, producing shear forces and shockwaves that act on cell membranes. This action eventually breaks the cell membranes apart.

 

Ultrasonic cell disruptors work with a wide range of sample types such as bacteria, yeast, animal cells and plant cells. They efficiently break down cell walls and membranes to release intracellular contents with good purity.

 

Users can adjust key parameters including ultrasonic frequency, output power and treatment duration to fine-tune the lysis outcome.

 

 

 

1. Extract intracellular biomolecules including enzymes, proteins and nucleic acids

2. Prepare bacterial lysates used in vaccine manufacturing

3. Isolate intracellular organelles such as mitochondria and lysosomes

4. Prepare cell membrane fractions for biofilm studies

 

 

 

1. Frequency selection

For plant cell disruption, ultrasonic frequencies between 20–50 kHz are generally suitable.

Frequencies below 20 kHz tend to damage intracellular organelles, while frequencies above 100 kHz deliver poor disruption efficiency.

 

2. Frequency and power correlation

Higher ultrasonic frequencies typically require lower power to achieve adequate cell disruption.

Lower frequencies demand higher power to reach equivalent lysis results.

 

3. Frequency and selective disruption

Ultrasound at different frequencies targets distinct cellular structures preferentially.

Lower frequencies work better for breaking cell walls, whereas higher frequencies are ideal for rupturing cell membranes.

 

4. Frequency and sample temperature rise

Higher-frequency ultrasound helps minimize excessive sample heating.

Conversely, low-frequency ultrasound usually causes significant temperature increases, so active cooling is required.

 

In summary, proper frequency selection is critical for ultrasonic disruption of plant cells. The 20–50 kHz range is the recommended starting point. Users should further optimize frequency and power settings according to their specific target cell samples.

 

       

 

 

 

 

 

 

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